Oxidative Stress in Oral Mucosa Cells of Adults with Fixed Orthodontic
Keywords:
orthodontic treatment., cytotoxicity, oxydative stress, catalase, SOD, fixed appliancesAbstract
Objective: The aim of our study was to assess the level of cytotoxicity of orthodontic appliances by checking the hypothesis that they induce an oxidative stress in mucosal cells.
Methods: Our study included two groups: 29 controls and 34 patients undergoing orthodontic treatment with fixed appliances. Samples were collected before bonding (T0), after one month (T1), and after three months (T2) of treatment.
Results: Results indicate the presence of oxidative stress following bonding, with significant differences in catalase activity (p = 0.039 at T1; p = 0.01 at T2) and in SOD activity (p = 0.001 at T1 and T2). The highest levels of enzymatic activities were recorded at T1 for both enzymes but subsided at T2, suggesting cellular self-repair capabilities in response to orthodontic alloys.
Conclusion: In view of our results, an appropriate choice of orthodontic alloys is required. Moreover, an identification of the subjects at risk for developing corrosion and galvanism and more attention are required to avoid ionic release in the oral cavity.
References
Dwivedi, Tikku, Khanna, Maurya, Verma, Murthy (2020) Effect of appliance type on salivary parameters during treatment. 90(4), 532-538.
Spalj, Mlacovic Zrinski, Tudor Spalj, Ivankovic Buljan (2015) Release of nickel and chromium ions in the saliva of patients with fixed orthodontic appliance: An in-vivo study. 6(1), 62.
Kao, Ding, He, Chou, Huang (2012) In-vitro assessment of oxidative stress generated by orthodontic archwires. 141(5), 583-9.
Natarajan, Padmanabhan, Chitharanjan, Narasimhan (2007) Cytotoxicity of orthodontic wire corroded in fluoride solution in vitro. 77(2), 349-54.
Montanaro, Cervellati, Campoccia, Prati, Breschi, Arciola (2005) No genotoxicity of a new nickel-free stainless steel. 28(1), 58-65.
Ricardo Carvalho Bueno, Roberta Tarkany Basting (2015) In vitro study of human osteoblast proliferation and morphology on orthodontic mini-implants. 85(6), 920-6.
Westphalen, Menezes, Prá (2008) In vivo determination of genotoxicity induced by metals from orthodontic appliances using micronucleus and comet assays. 7(4), 1259-66.
Sudha Halkai, Anand Mangalgi, Kundan Shah, Ganesh Paramshivam, Deepika Mallasure (2017) Comparison of metal release from fixed orthodontic appliances in oral mucosa cells in patients with and without fixed orthodontic appliances- An in vivo study. 5(2), 142-147.
Nia, Van Straaten, Godschalk (2000) Immunoperoxidase detection of polycyclic aromatic hydrocarbon-DNA adducts in mouth floor and buccal mucosa cells of smokers and nonsmokers. 36(2), 127-33.
Bradford (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of proteindye binding. 72, 248–254.
Marklund, Marklund (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. 47, 469–474.
Aebi (1984) Catalase in vitro. 105, 121–126.
T. S. Gonçalves, L. M. Menezes, Trindade, et al. (2014) Cytotoxicity and genotoxicity of orthodontic bands with or without silver soldered joints. 762, 1-8.
E. Fernandez-Minano, C. Ortiz, A. Vicente, J. L. Calvo Guirado, A. J. Ortiz (2011) Metallic ion content and damage to the DNA in oral mucosa cells of children with fixed orthodontic appliances. 24(5), 935.
F. Heravi, M. R. Abbaszadegan, M. Merati, N. Hasanzadeh, E. Dadkhah, F. Ahrari (2013) DNA damage in oral mucosa cells of patients with fixed orthodontic appliances. 10(6), 494-500.
A. Martin-Camean, Jos A, P. Mellado-Garcia, A. Iglesias-Linares, E. Solano, A. M. Camean (2015) In vitro and in vivo evidence of the cytotoxic and genotoxic effects of metal ions released by orthodontic appliances: A review. 40(1), 86-113.
P. Tomakidi, U. Koke, R. Kern, et al. (2000) Assessment of acute cyto- and genotoxicity of corrosion eluates obtained from orthodontic materials using monolayer cultures of immortalized human gingival keratinocytes. 61(1), 2-19.
H. S. Hafez, E. M. Selim, F. H. Kamel Eid, W. A. Tawfik, E. A. Al-Ashkar, Y. A. Mostafa (2011) Cytotoxicity, genotoxicity, and metal release in patients with fixed orthodontic appliances: a longitudinal in-vivo study. 140(3), 298-308.
F. Faccioni, P. Franceschetti, M. Cerpelloni, M. E. Fracasso (2003) In vivo study on metal release from fixed orthodontic appliances and DNA damage in oral mucosa cells. 124(6), 687-94.
P. Buczko, I. Szarmach, M. Grycz, I. Kasacka (2017) Caspase-3 as an important factor in the early cytotoxic effect of nickel on oral mucosa cells in patients treated orthodontically. 55(1), 37-42.
OZTURK Firat (2012) Genotoxic effects of banding procedure with different orthodontic cement on human oral mucosa cells. 42, 1157-1165.
G. Rahilly, N. Price (2003) Nickel allergy and orthodontics. 30(2), 171-4.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Authors and Global Journals Private Limited
This work is licensed under a Creative Commons Attribution 4.0 International License.
